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Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Our products enable scientists to improve productivity and efficiency in scientific discovery and research.

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Dec 11, 2019 · FreeSolv: calculated and experimental hydration free energies (kcal/mol) for 642 small neutral molecules in water. Lipophilicity: experimental data on octanol/water distribution coefficient (logD at pH 7.4) for 4200 compounds. All these datasets are available in the validation…

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Soy protein (New Fujipro-E; Fuji Oil, Osaka, Japan) was hydrolyzed by porcine pepsin (activity; 1:10,000, Nacalai Tesque, Kyoto, Japan) at pH 2.0 and 37°C for 24 h. Porcine pepsin (1 g/100 g) was added to the protein. The digest was heated at 90°C for 30 min and neutralized with 2 mol/L NaOH. The digest was centrifuged at 4500 × g for 20 min.

Quantity Value Units Method Reference Comment; Δ r H°: 125. ± 10. kJ/mol: N/A: Caldwell, Rozeboom, et al., 1984: gas phase; Reanchored to average data from Paul and Kebarle, 1990 and Meot-ner and Sieck, 1986.; value altered from reference due to change in acidity scale; B,M: Δ r H°: 99.6

Oct 12, 2018 · Edible portions of bananas contain high levels of polyphenol oxidase, which catalyzes reactions in the melanin formation pathway. Tyrosine, a physiological substrate of polyphenol oxidase, has an analogous structure to acetaminophen. We investigated whether banana extract causes structural changes in acetaminophen and a decrease in its potency.

Nov 06, 2007 · Despite having irregular structure, protein loops often adopt specific conformations that are critical to protein function. Most studies in de novo protein design have focused on creating proteins with regular elements of secondary structure connected by very short loops or turns. To design longer protein loops that adopt specific conformations, we have developed a protocol within the Rosetta ...

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Feb 06, 2019 · Then, experiments were performed under the same settings after an acclimatization period. Participants wore a newly developed heated humidification mask for 10 to 20 minutes, which is produced by Kao Corporation (Tokyo, Japan), or non-heated-humidification mask (placebo). The heated humidification mask has a 3-dimensional structure.

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Since 1875, Shimadzu is pursuing leading-edge science and technologies in analytical and measuring instruments including chromatographs and mass spectrometers, medical devices, aeronautics, and industrial equipment.

Global Reach; Intertek is the industry leader with over 46,000 people in 1,000 locations in over 100 countries. Whether your business is local or global, we can ensure your products meet quality, health, environmental, safety, and social accountability standards for virtually any market around the world.

E 1 =E o - 0.05916 • pH outer. in the same way E 2 can be defined as: E 1 =E o - 0.05916 • pH inner. Since E 1 and E 2 in the pH measuring loop are of opposite polarities the equation becomes: E t = E 2 - E 1 E t = 0.05916 (pH inner - pH outer) (12)

Sep 15, 2020 · The protein integrity profile of free BSA before and after exposure to simulated gastric fluid (SGF; 2 g NaCl/1L water, pH 1.2, with or without 3.2 g/L pepsin) or simulated intestinal fluid (SIF; 6.8 g KH 2 PO 4 /1 L water, pH 6.8, with or without 10 g/L pancreatin) is shown in Additional file 1: Fig. S1.

The liquid was washed with low-speed table centrifuge to neutral pH value after being cooled to room temperature. The precipitate was dried in an air dryer at 35 °C to obtain carboxylated g-C 3 N 4 nanosheets. Preparation of N–C QDs. Wheat straw was crushed, dried for 6 h at 80 °C, and added with 3 mol/L NaOH solution in a 100 mL bottle.

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Nov 27, 2020 · The H 2 permeability of the Zirfon separator was as high as 20 × 10 −12 mol cm −1 s −1 bar −1, as shown in Figure 4a. On the contrary, the H 2 permeability of the C20 and C50 separators was 8.38 ± 0.48 × 10 −12 mol cm −1 s −1 bar −1 and 0.69 ± 0.18 × 10 −12 cm −1 s −1 bar −1, respectively.

Nitrite and nitrate are main stable products of nitric oxide, a pivotal cellular signaling molecule, in biological fluids. Therefore, accurate measurement of the two ions is profoundly important. Nitrite is difficult to be determined for a larger number of interferences and unstable in the presence of oxygen. In this paper, a simple, cost-effective and accurate HPLC method for the ...

Nov 06, 2007 · Despite having irregular structure, protein loops often adopt specific conformations that are critical to protein function. Most studies in de novo protein design have focused on creating proteins with regular elements of secondary structure connected by very short loops or turns. To design longer protein loops that adopt specific conformations, we have developed a protocol within the Rosetta ...

Mar 01, 2001 · Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters ...

Soy protein (New Fujipro-E; Fuji Oil, Osaka, Japan) was hydrolyzed by porcine pepsin (activity; 1:10,000, Nacalai Tesque, Kyoto, Japan) at pH 2.0 and 37°C for 24 h. Porcine pepsin (1 g/100 g) was added to the protein. The digest was heated at 90°C for 30 min and neutralized with 2 mol/L NaOH. The digest was centrifuged at 4500 × g for 20 min.

Quantity Value Units Method Reference Comment; Δ r H°: 125. ± 10. kJ/mol: N/A: Caldwell, Rozeboom, et al., 1984: gas phase; Reanchored to average data from Paul and Kebarle, 1990 and Meot-ner and Sieck, 1986.; value altered from reference due to change in acidity scale; B,M: Δ r H°: 99.6

Introduction. Molecular clock (Zuckerkandl and Pauling 1965) is the constancy of evolutionary rates across lineages.This constancy has been widely used in determining the divergence time from nucleotide or amino acid sequences (Vawter et al. 1980; Hasegawa et al. 1985; Bromham and Penny 2003; Peterson et al. 2004), and its theoretical basis is the neutral theory of molecular evolution (Kimura ...

Validation Data Gallery WB analysis using 21728-1-AP Various lysates were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Oct 12, 2018 · Edible portions of bananas contain high levels of polyphenol oxidase, which catalyzes reactions in the melanin formation pathway. Tyrosine, a physiological substrate of polyphenol oxidase, has an analogous structure to acetaminophen. We investigated whether banana extract causes structural changes in acetaminophen and a decrease in its potency.

The molecular triggers of sexual differentiation into gametocytes by blood stage Plasmodium falciparum , the most malignant human malaria parasites, are subject of much investigation for potential transmission-blocking strategies. The parasites are readily grown in vitro with culture media supplemented by the addition of human serum (10%) or by a commercially available substitute (0.5% AlbuMAX).

The editors of JAMA recognize the challenges, concerns, and frustration about the shortage of personal protective equipment (PPE) that is affecting the care of patients and safety of health care workers in the US and around the world. We seek creative immediate solutions for how to maximize the use of PPE, to conserve the supply of PPE, and to identify new sources of PPE.

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